| 摘要: |
| 为分析宠物源大肠杆菌对抗菌药物的耐药情况及ESBLs耐药基因型，采集乌鲁木齐市181份宠物犬、猫肛拭子样品，分离获得120株大肠杆菌，通过Kirby-Bauer(K-B)药敏纸片法对分离株进行药物敏感性试验，采用PCR方法检测CTX-M(CTX-M-1G、CTX-M-2G和CTX-M-9G)、TEM和SHV三种超广谱β-内酰胺酶(ESBLs)基因。结果显示:120株大肠杆菌对四环素、氨苄西林、复方新诺明和哌拉西林的耐药率在35.8%~60.8%；对头孢噻肟、链霉素、庆大霉素、氯霉素、环丙沙星和多粘菌的耐药率在10.0%~29.2%；对亚胺培南、左氧氟沙星、阿米卡星、头孢他啶、氨曲南、氨苄西林-舒巴坦、阿莫西林、头孢吡肟和美罗培南的耐药率在0.8%~10.0%。58株(48.3%)大肠杆菌呈多重耐药表型(Multidrug resistance，MDR)；犬源分离株主要对3类抗生素产生耐药(16.9%，12/71)，猫源分离株主要对4类抗生素产生耐药(18.4%，9/49)；其中共检测出35株ESBLs阳性菌株，CTX-M-1G、CTX-M-9G和TEM基因的检出率分别为40.0%(14/35)、28.6%(10/35)和34.3%(12/35)，未扩增出CTX-M-2G和SHV基因。综上，从健康和患病宠物中均分离到多重耐药大肠杆菌，流行的ESBLs基因型以CTX-M和TEM为主。 |
| 关键词: 宠物 大肠杆菌 耐药性 ESBLs 检测 |
| DOI：10.11841/j.issn.1007-4333.2020.11.10 |
| 分类号: |
| 基金项目:新疆维吾尔自治区自然科学基金(2019D01A51)；自治区高层次人才引进工程项目 |
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| Drug resistance analysis and ESBLs genotype detection of Escherichia coli derived from pet dogs and cats feces in Urumqi |
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TONG Panpan,YANG Yinping,XIE Jinxin,ZHANG Mengmeng,ZHANG Ling,TANG Xuelin,ZHANG Yi,LU Xing,SU Zhanqiang
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College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China
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| Abstract: |
| In order to identify the antibiotic resistance of Escherichia coli and ESBLs genes from pets, a total of 120 stains were isolated from 181 rectal swabs from dogs and cats in Urumqi. The drug sensitivity of the isolates was investigated by Kirby-Bauer(K-B)method. Three different extended-spectrum β-lactamases(ESBLs)of CTX-M(CTX-M-1G, CTX-M-2G and CTX-M-9G), TEM and SHV genes were detected by PCR. The results showed that: The drug resistance rate of the 120 strains of E. coli to tetracycline, ampicillin, compound minocycline and piperacillin was 35. 8%-60. 8%, that to cefotaxime, streptomycin, gentamicin, chloramphenicol, ciprofloxacin, and polymyxin was 10. 0%-29. 2%, and that to imipenem, levofloxacin, amikacin, ceftazidime, aztreonam, ampicillin-sulbactam, amoxicillin, cefepime, and meropenem was 0. 8%-10. 0%. A total of 58 strains(48. 3%)of E. coli showed multidrug resistance(MDR). The strains from dogs were mainly resistant to 3 kinds of antibiotics(16. 9%, 12/71), while the strains from cats were mainly resistant to 4 kinds of antibiotics(18. 4%, 9/49). Among them, 35 ESBLs positive strains were detected. The detection rates of ESBLs gene CTX-M-1G, CTX-M-9G and TEM were 40. 0%(14/35), 28. 6%(10/35)and 34. 3%(12/35), respectively. CTX-M-2G and SHV were not detected. In conclusion, MDR E. coli strains were isolated from healthy and sick pets and the CTX-M and TEM genes were most popular ESBLs genotypes. |
| Key words: pet Escherichia coli resistance ESBLs detection |
