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| 酸马奶中乳酸菌对Escherichia coli O8腹泻小鼠生长性能、血清免疫及抗氧化指标的影响 |
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吉米斯1,敖日格乐1*,王纯洁2,斯木吉德1,高瑞娟2,阿日查1,超克1,张剑1
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1.内蒙古农业大学 动物科学学院, 呼和浩特 010018;2.内蒙古农业大学 兽医学院, 呼和浩特 010018
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| 摘要: |
| 为研究酸马奶中乳酸菌(Lactobacillus paracasei subsp. tolerans)对大肠杆菌腹泻小鼠生长性能、细胞因子水平和抗氧化酶活性的影响。选择体重为(20±2)g的60 只SPF级昆明小鼠，随机分为6 组，每组10 只，雌雄各半。通过腹腔注射致病性Escherichia coli O8菌悬液制备小鼠腹泻模型，并用不同浓度的乳酸菌菌悬液和抗生素进行治疗，检测其对腹泻小鼠生长性能、免疫器官指数、血清免疫指标及抗氧化指标的影响。其中4 组分别灌胃0.3 mL活菌数为1×106、1×108、1×1010 CFU/mL的LAB菌悬液和0.15 g/kg体重的盐酸环丙沙星；阴性对照组灌胃生理盐水，并不进行治疗；正常对照组不做任何处理。试验期为7 d。结果表明:灌胃不同剂量LAB对小鼠生长性能具有促进作用(P<0.05)；灌胃1×1010 CFU/mL LAB菌悬液可显著提高小鼠脾脏指数(P<0.05)，较阴性对照组提高了53.91%；小鼠胸腺指数呈上升趋势，但差异不显著(P>0.05)；乳酸菌可显著提高IL-4、IL-10、TGF-β 水平，降低IL-6、IFN-γ水平(P<0.05)，LAB高剂量组效果最佳；可显著提高CAT、T-AOC、SOD和GSH-Px活力，显著降低MDA含量(P<0.05)，高剂量组效果最优。综上，腹泻小鼠灌胃乳酸菌能够促进小鼠免疫器官发育，并能调节血清免疫和抗氧化指标。 |
| 关键词: 酸马奶 乳酸菌 腹泻 SPF KM小鼠 生长性能 细胞因子 抗氧化酶 |
| DOI：10.11841/j.issn.1007-4333.2020.01.13 |
| 分类号: |
| 基金项目:国家自然科学基金项目(31660677)；内蒙古自治区科技计划项目(201602051) |
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| Effects of lactic acid bacteria in koumiss on the growth performance, serum immunity and antioxidant indexes of mice with diarrhea induced by Escherichia coli O8 |
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Jimisi1,Aorigele1*,WANG Chunjie2,Simujide1,GAO Ruijuan2,Aricha1,CHAO Ke1,ZHANG Jian1
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1.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China
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| Abstract: |
| This study was designed to investigate the effects of lactic acid bacteria in koumiss(Lactobacillus paracasei subsp. tolerans)on the growth performance, cytokine levels and antioxidant enzyme activities of mice with diarrhea caused by Escherichia coli. Sixty SPF Kunming mice weighing(20+2)g were randomly divided into 6 groups with 10 mice in each group, half male and half female. The diarrhea model of mice was established by intraperitoneal injecting pathogenic E. coli O8 bacterial suspension, and then treated with different concentrations of lactic acid bacteria and antibiotics to test its growth performance, immune organ index and serum immune index and antioxidant index. Four groups were intragastrically administered with 0. 3 mL of live LAB bacterial suspension of 1×106, 1×108, 1×1010 CFU/mL and 0. 15 g/kg of ciprofloxacin hydrochloride, respectively. Mean while, the negative control group was injected with normal saline only without any treatment afterwards, and the normal control group did not give any treatment. The experimental period took 7 days. The results showed that: The intragastrically administered different doses LAB promoted the growth performance of mice(P<0. 05); The spleen index of mice was significantly increased by 1×1010 CFU/mL LAB bacteril suspension(P<0. 05), which was 53. 91% higher than that of negative control group. An upward trend was presented by the thymus index of mice, but the difference is not significant(P>0. 05); There were significant increase in the levels of IL-4, IL-10 and TGF-β, and decrease in the levels of IL-6 and IFN-γ(P<0. 05), and the high dose group of LAB displayed the best effect. There were significantly increase in the activities of CAT, T-AOC, SOD and GSH-Px, significant decrease in the level of MDA content(P<0. 05), and the high dose of LAB group showed optimal effect. In conclusion, the intragastric administration of lactic acid bacteria in diarrhea mice could promote the development of immune organs and regulate serum immunity and antioxidant indexes. |
| Key words: koumiss lactic acid bacteria diarrhea SPF KM mice growth performance cytokines antioxidant enzymes |
