| 摘要: |
| 为明确美丽组尖孢镰孢菌和李瑟组镰孢菌种间和种内的差异与亲缘关系，以采自不同寄主的57株镰孢菌（Fusarium）为研究对象，采用ISSR-PCR标记技术对其进行遗传多样性分析，结果表明：利用11条引物对供试的3种菌株进行ISSR-PCR扩增，共扩增出121条条带，其中多态性位点为117个，多态性比例为96.7%，平均每条引物产生条带为11条。说明镰孢菌基因组DNA的SSR区域具有明显的多态性，镰孢菌的遗传多样性采用ISSR技术是可行的分析。聚类分析结果表明：57个菌株间的遗传相似系数为0.568~0.992，当相似系数为0.568时，供试菌株可明显分为两大类群，第一类群IG-I为1~35，全部为美丽组尖孢镰孢菌，第二类群IG-Ⅱ为36~57，全部为李瑟组镰孢菌；第二类群又可以分为两大亚类群，第一亚类为轮枝镰孢菌，第二亚类为层生镰孢菌。ISSR类群划分与镰孢菌菌种分类之间具有一定的相关性，而与其地理来源无相关性。本研究镰孢菌种间与种内均表现出明显的遗传差异。 |
| 关键词: 镰孢菌 遗传多样性 ISSR技术 形态学分类 |
| DOI：10.11841/j.issn.1007-4333.2019.05.12 |
| 分类号: |
| 基金项目:山西省重点研发项目（201703D221010-1） |
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| Genetic diversity of 57 Fusarium strains based on ISSR analysis |
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SUN Changqing1, QU Fei1, WANG Jin1, YUN Xiaodong1, LI Xinfeng2
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1.Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;2.College of Agriculture, Shanxi Agricultural University, Taigu 030801, China
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| Abstract: |
| In order to understand the genetic difference and phylogenic relationship within and between the Fusarium species in Fusarium oxysporum and Lee harp Fusarium group,the genetic diversity of 57 Fusarium strains separated from different disease plants was examined by inter-simple sequence repeats (ISSR).A total of 11 primers were used to amplify three Fusarium isolates.The results showed that 121 fragments were amplified,among which the number of polymorphic loci was 117 accounting for 96.7% of the total amplified fragments.Each primer produced 11 fragments on average.The genetic diversity was analyzed according to the amplified results and the ISSR analysis was adopted to investigate the genetic diversity.A total of 57 Fusarium strains showed genetic similarity coefficient between 0.568-0.992.When the genetic similarity coefficient was set at 0.568,these tested strains could be clearly divided into two groups:The first group included No.1 to 35 straines,which belonged to F.oxysporum;The second group included No.36 to 57 strains,which belonged to Lee harp Fusarium group.The second group had 2 subgroups:The first subgroup was F.verticillioides,and the second subgroup was F.proliferatum.There was a certain level correlation between the classification of ISSR groups and the classification of Fusarium strains,and there was no correlation with the geographical origin of the strains.In conclusion,the 57 Fusarium strains showed significant genetic differences between species and within species. |
| Key words: Fusarium genetic diversity ISSR technology morphological taxonomy |
