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| CYP11A1、CYP17A1、CYP19A1基因在小尾寒羊下丘脑-垂体-卵巢轴的表达 |
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田志龙1,2, 刘秋月1, 王翔宇1, 狄冉1, 胡文萍1, 王玉琴2, 张效生3, 张金龙3, 储明星1
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1.中国农业科学院 北京畜牧兽医研究所/农业部动物遗传育种与繁殖重点实验室, 北京 100193;2.河南科技大学 动物科技学院, 河南 洛阳 471003;3.天津市畜牧兽医研究所, 天津 300381
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| 摘要: |
| 为揭示类固醇生成相关基因CYP11A1、CYP17A1、CYP19A1在绵羊多羔繁殖中的作用，以不同繁殖力小尾寒羊为研究对象，采用实时荧光定量PCR技术检测CYP11A1、CYP17A1、CYP19A1基因在小尾寒羊大脑、小脑、下丘脑、垂体、子宫、卵巢、输卵管中的表达。结果表明：CYP11A1基因主要在小尾寒羊卵巢、输卵管、子宫内表达；CYP11A1基因在单羔小尾寒羊卵巢的表达极显著低于多羔群体（P<0.01），在子宫的表达量极显著高于多羔群体（P<0.01）；CYP17A1基因主要在小尾寒羊卵巢、输卵管、下丘脑表达；CYP17A1基因在多羔小尾寒羊群体下丘脑、子宫的表达量显著低于单羔群体（P<0.05）。CYP19A1基因主要在小尾寒羊卵巢、下丘脑表达。本究结果提示CYP11A1、CYP17A1基因可能参与小尾寒羊多羔性状的调控。 |
| 关键词: 小尾寒羊 多羔 CYP11A1 CYP17A1 CYP19A1 组织表达 |
| DOI：10.11841/j.issn.1007-4333.2019.04.11 |
| 分类号: |
| 基金项目:国家自然科学基金项目（31772580）；国家肉羊产业技术体系专项（CARS-38）；中国农业科学院科技创新工程（ASTIP-IAS13） |
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| Expression of CYP11A1, CYP17A1 and CYP19A1 genes in hypothalamus-pituitary-ovary axis of Small Tail Han sheep |
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TIAN Zhilong1,2, LIU Qiuyue1, WANG Xiangyu1, DI Ran1, HU Wenping1, WANG Yuqin2, ZHANG Xiaosheng3, ZHANG Jinlong3, CHU Mingxing1
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1.Institute of Animal Science/Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2.College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;3.Tianjin Institute of Animal Sciences, Tianjin 300381, China
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| Abstract: |
| To reveal the roles of CYP11A1,CYP17A1 and CYP19A1 genes in the high fecundity of sheep,Real-time fluorescence quantitative PCR (RT-qPCR) was performed to detect CYP11A1,CYP17A1 and CYP19A1 mRNA expressions in seven different tissues (brain,cerebellum,hypothalamus,pituitary,uterus,ovary and oviduct) of multiparous and uniparous Small Tail Han sheep.The results showed that CYP11A1 was expressed in ovary,oviduct and uterus of Small Tail Han sheep.The expression of CYP11A1 in ovary of multiparous Small Tail Han sheep was extremely significantly higher than that of uniparous Small Tail Han sheep (P<0.01).However,the expression of CYP11A1 in the uterus of multiparous Small Tail Han sheep was extremely significantly lower than that of uniparous Small Tail Han sheep (P<0.01).The CYP17A1 gene was mainly expressed in ovary,oviduct and hypothalamus of Small Tail Han sheep.The expression of CYP17A1 in hypothalamus,uterus of multiparous Small Tail Han sheep was significantly lower than that of uniparous Small Tail Han sheep (P<0.05).The CYP19A1 gene was mainly expressed in ovary and hypothalamus of Small Tail Han sheep.In conclusion,the results suggested that CYP11A1 and CYP17A1 might be involved in the regulation of multiple births in Small Tail Han sheep. |
| Key words: Small Tail Han sheep prolificacy CYP11A1 CYP17A1 CYP19A1 tissue expression |
