| 摘要: |
| 本试验采用组织培养方法诱导核桃叶柄体细胞胚胎发生,并对不同条件下产生的愈伤组织进行了细胞学观察。结果证明,核桃叶柄体细胞胚胎发生合适的培养基为:DKW 附加KT 2.0 mg/L、IBA 0.02 mg/L、谷氨酰胺250 mg/L、水解酪蛋白100 mg/L、20%硫代硫酸钠5 mL/L、蔗糖30 g/L、琼脂7 g/L。外植体接种方向以叶柄形态学上端露在空气中下端埋入培养基为宜。显微观察证明胚性细胞内出现大最油滴,非胚性细胞内则无油滴出现。 |
| 关键词: 核桃叶柄 体细胞胚胎发生 细胞观察 脂肪 |
| DOI： |
| 分类号:Q945.4 |
| 基金项目:国家863计划“人工种子”专题资助 |
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| Somatic Embryogenesis and Cytological Observation from Petiole of Walnat(Juglans regia L.) |
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Liu Shulan Han Biwen (Beijing Agricultural University),Chen Zhenghua (Institute of Genetics,Academia Sinica)
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| Abstract: |
| Somatie embryogenesis of walnut(Juglans regia L.)was induced by tissue culture methods and cytological observations were conducted.The result showed that DKW medium supplanted with KT 2.0mg/L,IBA 0.02mg/L, glutamine(Gln)250mg/L,caseni hybrolyzte 100mg/L,20% sodium thiosulfate 5mL/L,sacrose 30g/L and agar 7g/L was the most suitable for somatik embryo- genesis of walnut petiole.After 21 days of inoculation the somatic embryos had been formed on the explants with the apical end exposed to the air and the basal... |
| Key words: petiole of walnut somatic embryogenesis cell observation fat |
