| 摘要: |
| 为探究在非生物逆境胁迫条件下免疫亲和素（immunophilins）基因家族在水稻（Oryza sativa L. japonica Nipponbare）中的功能，利用生物信息学技术鉴定水稻中免疫亲和素基因家族成员，并进行克隆及组织特异性、非生物逆境胁迫下的表达分析。结果表明，水稻基因组中存在30个OsFKBPs及29个OsCYPs免疫亲和素家族基因。30个OsFKBPs主要聚类为3个大的亚群，29个OsCYPs主要聚类为4个大的亚群。通过PCR扩增得到完整的OsFKBPs与OsCYPs基因编码序列，且序列信息与预测一致。OsFKBPs与OsCYPs基因在水稻的各个组织、各个发育时期均有不同程度的表达。OsFKBP19在各组织各发育时间表达量较为一致；OsCYP29在叶鞘中表达量最高而OsCYP57在根中表达量最高。定量PCR结果显示，在高光强胁迫处理4 h后水稻叶片中OsCYP37基因被诱导持续上升表达，而在渗透胁迫及盐胁迫处理中无显著变化；高光强及渗透胁迫处理4 h后水稻叶片中OsFKBP19持续上升表达，而在盐胁迫处理中波动上升表达。 综上，OsFKBPs及OsCYPs参与水稻的高光强、渗透胁迫及盐胁迫应答过程。 |
| 关键词: 水稻 免疫亲和素 基因家族 非生物胁迫 表达谱 |
| DOI：10.11841/j.issn.1007-4333.2024.05.02 |
| 投稿时间：2023-10-27 |
| 基金项目:杂交水稻国家重点实验室开放课题（KF202204）；安徽省高等学校科学研究项目（2022AH051034，2022AH051048）；安徽省优秀人才项目（GXFX2023015） |
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| Cloning of the immunophilins gene family and analysis of its expression pattern under abiotic stress conditions |
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JIANG Zhuanzhuan, PAN Jun, WU Juan
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| (College of Life Sciences/Provincial Key laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui，Anqing Normal University， Anqing 246133， China) |
| Abstract: |
| To explore the function of the immunophilins gene family in rice （Oryza sativa L. japonica Nipponbare） under abiotic stress conditions， the immunophilins gene family members in rice were identified by bioinformatics techniques， followed by cloning， tissue and abiotic stress expression analysis. The results showed that there are 30 OsFKBPs and 29 OsCYPs immunophilins family genes in rice genome. The 30 OsFKBPs were mainly clustered into three large subgroups. The 29 OsCYPs were mainly clustered into four large subgroups. The coding sequences of OsFKBPs and OsCYPs genes were obtained through PCR amplification， and the sequence information was consistent with the predicted in the database. Expression analysis revealed that OsFKBPs and OsCYPs genes exhibited distinct expression patterns in various tissues and developmental stages of rice， with certain genes showing tissue-specific expression. The expression of OsFKBP19 remained consistent across different tissues and developmental stages. OsCYP29 exhibited the highest expression in leaf sheath， while OsCYP57 showed the highest expression in roots. Quantitative PCR results revealed that the expression of the OsCYP37 gene in rice leaves was induced and continuously increased after 4 h of high light intensity stress， but displayed no significant changes under osmotic stress and salt stress. The expression of OsFKBP19 in rice leaves continued to increase after 4 h of high light intensity and osmotic stress， but fluctuated under salt stress. Taken together， OsFKBPs and OsCYPs play important roles in the regulation of high light intensity， osmotic stress and salt stress responses in rice. |
| Key words: rice immunophilins gene family abiotic stress expression profile |
