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小麦3种锈菌PCWDEs家族基因鉴定及表达分析
吴珂妍1,千慧敏1,赵俊溢1,李闯2,路亚南1,邢国珍1,刘娜1*,郑文明2*
0
(1.河南农业大学 生命科学学院,郑州 450002;2.省部共建小麦玉米作物学国家重点实验室,郑州 450002)
摘要:
为挖掘小麦锈菌侵染过程中发挥关键作用的植物细胞壁降解酶(Plant cell wall-degrading enzymes, PCWDEs)基因,通过生物信息学方法对小麦3种锈菌(叶锈菌、条锈菌和秆锈菌)基因组中的碳水化合物活性酶(Carbohydrate-active enzymes, CAZymes)进行预测分析,在此基础上对其中的PCWDEs基因家族进行全基因组范围的筛选鉴定,并利用qRT-PCR方法对叶锈菌PCWDEs家族成员在侵染过程中的转录水平进行了检测分析。结果表明:1)小麦叶锈菌、条锈菌和秆锈菌基因组分别编码355、322和345个CAZymes家族成员基因以及160、153和158个PCWDEs基因;小麦3种锈菌共含75个保守CAZymes基因亚家族和94个PCWDEs直系同源基因簇。2)16个叶锈菌特异性PCWDEs基因在亲和(Pt15-‘中国春’)/非亲和(Pt15-‘云麦34’)互作过程中均受到不同程度的诱导表达,但非亲和互作中在侵染更早期就已受到诱导上调表达。3)叶锈菌特异性PCWDEs基因簇中有4个PCWDEs被鉴定为类扩展蛋白,其在侵染过程中受到诱导表达,说明类扩展蛋白可能在降解纤维素突破侵染屏障中发挥重要作用。综上,在小麦3种锈菌全基因组范围鉴定了PCWDEs基因,初步明确部分基因在锈菌侵染过程中的表达模式,PCWDEs可能在小麦锈菌致病过程中发挥重要作用。
关键词:  小麦锈菌  PCWDEs  生物信息学分析  qRT-PCR  侵染机制
DOI:10.11841/j.issn.1007-4333.2024.04.10
投稿时间:2023-11-13
基金项目:国家重点研发计划(2021YFD1401000); 河南省重大科技专项(221100110100); 河南农业大学拔尖人才项目(30500924)
Identification and expression analysis of PCWDEs family genes in three wheat rust fungi
WU Keyan1, QIAN Huimin1, ZHAO Junyi1, LI Chuang2, LU Ya’nan1, XING Guozhen1, LIU Na1*, ZHENG Wenming2*
(1.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China;2.State Key Laboratory of Wheat and Maize Crop,Zhengzhou 450002, China)
Abstract:
In order to explore the key plant cell wall-degrading enzymes (PCWDEs) genes, which play critical roles in the infection of wheat rust, bioinformatics methods were used to identify and analyze the Carbohydrate-active enzymes (CAZymes) existed in the genomes of three wheat rust fungi including Puccinia triticina Pt, Puccinia striiformis f. sp. tritici Pst and Puccinia graminis f. sp. tritici Pgt). The PCWDEs gene family was screened and identified from these CAZymes genes. The expression patterns for selected PCWDEs members of Pt were analyzed by qRT-PCR. The results showed as follows: 1) The genomes of wheat rust fungi encode 355, 322 and 345 CAZymes family members, and contain 160, 153 and 158 PCWDEs family members, for PtPst, and Pgt, respectively. There are 75 conserved CAZymes gene subfamiles and 94 orthologous gene clusters of PCWDEs in three wheat rust fungi. 2) 16 PCWDEs of Pt unique genes were induced at differential levels in compatible (Pt15-‘Chinese Spring’) and incompatible (Pt15-‘Yunmai 34’) combinations, and some of them were up-regulated earlier in the incompatible combinations. 3) Four Pt unique PCWDEs belonging to one gene cluster of Pt were homologous to extension-like proteins, which were also regulated for transcriptional level during the infection. In summary, all PCWDEs of wheat rust fungi in genome wide were identified, and the transcriptional patterns of some candidates were detected. The results in this study indicated that PCWDEs might play an important role during the infection of rust fungi.
Key words:  wheat rust fungi  PCWDEs  bioinformatics  qRT-PCR  pathogenic mechanism