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| 天津某牧场奶牛乳房炎奶样与环境中细菌的分离与鉴定 |
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胡海燕1,2,刘慧敏2,3,孟璐2,3,郑楠2,3,王加启2,3,程建波1*
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| (1.安徽农业大学 动物科技学院, 合肥 230000;2.中国农业科学院 北京畜牧兽医研究所/农业农村部奶产品质量安全风险评估实验室(北京), 北京 100193;3.中国农业科学院 北京畜牧兽医研究所/动物营养学国家重点实验室, 北京 100193) |
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| 摘要: |
| 为了解天津某牧场奶牛乳房炎奶样及环境中细菌的种类和流行性，采用美国加州乳房炎(CMT)方法筛选6头乳房炎患病奶牛，通过传统培养方法对乳房炎奶样、患病牛畜舍及奶厅样品进行细菌分离与鉴定。结果显示，乳房炎奶样及环境样品中共分离鉴定出217株菌株，主要优势菌为芽孢杆菌属(Bacillus)(146/217株，67.28%)，乳房炎奶样中占比25%(3/12)，畜舍样品中占比为47.87%(45/94)，奶厅样品中占比为88.29%(98/111)。乳房炎奶样中主要鉴定出4株葡萄球菌属(Staphylococcus)；畜舍样品中共鉴定出10株Staphylococcus和8株肠杆菌属(Enterobacter)，新垫料与饲料中各鉴定出1株克雷伯氏菌属(Klebsiella)；在奶厅样品前药浴液与后药浴杯样品中分离鉴定出3株BMV7。综上，本研究探究了天津某牧场中主要流行的微生物种类，提出该牧场卫生管理防控薄弱环节，为牧场奶牛乳房炎防控与卫生管理提出建议与意见，为进一步研究乳房炎致病菌溯源奠定基础。 |
| 关键词: 奶牛 乳房炎奶 环境 细菌 分离 鉴定 |
| DOI：10.11841/j.issn.1007-4333.2021.03.08 |
| 投稿时间：2020-06-02 |
| 基金项目:国家重点研发计划(2016YFD0500503)；中国农业科学院科技创新工程(ASTIP-IAS12)；现代农业产业技术体系专项资金(CARS-36) |
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| Isolation and identification of mastitic milk and environment bacteria in a dairy farm in Tianjin |
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HU Haiyan1,2,LIU Huimin2,3,MENG Lu2,3,ZHENG Nan2,3,WANG Jiaqi2,3,CHENG Jianbo1*
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| (1.College of Animal Science and Technology, Anhui Agricultural University, Hefei 230000, China;2.Laboratory of Quality & Safety Risk Assessment for Dairy Products of China Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;3.State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China) |
| Abstract: |
| In order to understand the species and prevalence of bacteria in mastitc milk samples and environment in a dairy farm in Tianjin, six cows with mastitis were screened by California mastitis test(CMT)method. Bacteria were isolated and identified from mastitc milk samples, cattle living environment, livestock house milk hall samples by traditional culture method. The results showed that 217 strains were isolated and identified from mastitc milk samples and environmental samples. The dominant bacteria were Bacillus(146/217 strains, 67. 28%), mastitc milk accounted for 25%(3/12), barn samples accounted for 47. 87%(45/94), and dairy samples accounted for 88. 29%(98/111). A total of 4 strains of Staphylococcus were identified in mastitc milk samples, 10 strains of Staphylococcus and 8 strains of Enterobacter were identified in livestock barn samples, 1 Klebsiella strain bacteria was identified in the new litter and feed samples, and 3 stains of BMV7 were isolated and identified from the sample of front medicine bath and back medicine bath cup. In summary, this study explored the main prevalent microbial species in a dairy farm in Tianjin, and proposed the weaknesses of the health management and control of the farm, and put suggestions and opinions for dairy cow mastitis prevention and control and health management, and laid the foundation for further traceability of mastitis pathogens. |
| Key words: dairy cattle mastitic milk environment bacteria isolation identification |
