| 摘要: |
| 为研究低磷胁迫(LP)、正常条件(HP)2个处理对转Bt基因玉米和郑单958根系形态以及磷利用效率的影响.以转Bt基因玉米(Bt799)和受体郑单958为试验材料,进行温室水培试验.研究证明:1)低磷胁迫后Bt799的根长、根鲜重和根冠比升高幅度高于郑单958,就总根长、根系投影面积总和、根表面积、根平均直径、单位立方米总根长和根总容积而言,Bt799玉米的LP/HP是郑单958玉米LP/HP的1.13、1.19、1.33、1.18、1.14和1.55倍.2)低磷胁迫降低各叶位Bt蛋白量,根部除根基处1~7 cm Bt蛋白量上升以外,其余根系均下降.在正常处理下,Bt799全株和根系Bt蛋白总量为1 750和423 ng,低磷处理下Bt799分别为1 470和784 ng.3)低磷胁迫下2种玉米叶片和根系磷含量和磷积累量显著降低,各叶位磷占总磷比例显著下降,但根系磷占总磷比例反而升高.研究表明,Bt799通过根长、根表面积和根系直径等形态变化来优化其空间分布构型,吸收更多的磷元素,但向叶片转运磷元素的能力较弱. |
| 关键词: 低磷胁迫 转Bt基因玉米 Bt蛋白 根系形态 磷利用效率 |
| DOI：10.11841/j.issn.1007-4333.2015.03.03 |
| 投稿时间：2014-10-27 |
| 基金项目:农业部新品种培育重大专项(2013ZX08011) |
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| Effect of low phosphorus stress on Bt protein and P efficiencies in transgenic maize |
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SHEN Ting-ting, LI Jin, SU He, ZHAN Yan-ting, PU Jun, DONG Xue-hui
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| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| To clarify the effects of low-phosphorous on Bt protein,root morphology and efficiencies,we took Bt799 and the back-ground ZhengDan958 as experimental materials to carry out hydroponics test.The results showed that:1)Root length,root fresh,weight and root shoot ratio of Bt 799 rose more than those of ZhengDan958 under P-deficient condition.The ratio of P-dificient and P-sufficient (LP/HP) of Bt 799 were 1.13、1.19、1.33、1.18、1.14 and 1.55 times higher than LP/HP (Zhengdan 958) in root total length,root projected area,root surface area,root average diameter,root total length per cubic meter and root volume.2)The leaf Bt protein expression content decreased,the same as root except 1-7 cm root base.Total Bt protein content of entire Bt799 and root were 1 750 and 423 ng under P-sufficient,while under P-deficient condition they were 1 470 and 784 ng.3)In different positions of both maize,P concentration and P accumulation decreased dramatically under P-deficient,the percentage of leaf P amount to total P amount decreased significantly,root showed the opposite trend.We conclude that Bt 799 optimized the spatial distribution of root to absorb more P element through the changes of morphological characteristics such as root length,root surface area,root diameter and so on,but the P transport ability to upper leaf is weak. |
| Key words: P-deficient Bt799 Bt protein root morphology P efficiencies |
