| 摘要: |
| 为选育矮化和耐缺铁的苹果砧木，配置了小金海棠×M9杂交组合，首先筛选M9纯合显性SSR分子标记并利用该标记鉴定小金海棠×M9的有性杂种后代，之后利用流式细胞术分析杂交后代中的倍性分离。结果表明：SSR标记CH02d12-160可作为鉴定杂种后代的特异标记。在1 285株小金海棠×M9实生苗中，有284株为有性杂种后代，其余1 001株为小金海棠无融合生殖后代，无融合生殖率为77.90%。在284株小金海棠×M9有性杂种后代中，138株的样品得到可靠倍性鉴定结果，其中2x、3x、4x和5x分别为5(3.62%)、21(15.22%)、63(45.65%)和49株(35.51%)。 |
| 关键词: 小金海棠 SSR 杂种 流式细胞术 |
| DOI：10.11841/j.issn.1007-4333.2014.05.11 |
| 投稿时间：2013-12-20 |
| 基金项目:公益性行业（农业）科研专项（201203075）；国家科技支撑计划项目（2013BAD02B01）；国家“863”计划项目（2011AA100204）；国家现代苹果产业技术体系（CARS-28） |
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| Hybrids identification and ploidy analysis of Malus xiaojinensis × M9 progeny |
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WANG Ran, WANG Yi, ZHANG Xin-zhong, HAN Zhen-hai
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| (College of Agronomy and Biotechnology/Institute for Horticultural Plants, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| Malus xiaojinensis is a apomictic tetraploid species with elite tolerance to low iron stress.In a breeding program for dwarfing and Fe-deficiency tolerant apple rootstocks, M.xiaojinensis was pollinated with M9.Homozygous dominant simple sequence repeat (SSR) marker was selected and applied in identification of hybrids derived from M.xiaojinensis × M9 and flow cytomery (FCM) was used to analyze the ploidy of the hybrids.CH02d12-160 was an available marker for hybrid identification.There were 284 hybrids and 1 001 apomictic-derived progenies among 1 285 seedlings.Thus the apomictic rate of this cross was 77.90%.The reliable data of ploidy were obtained for 138 seedlings out of the 284 hybrids.The numbers of 2x, 3x, 4x and 5x seedlings were 5(3.62%), 21(15.22%), 63(45.65%) and 49(35.51%) respectively. |
| Key words: Malus xiaojinensis simple sequence repeat hybrids flow cytometry |
