| 摘要: |
| 为了解EPS的粒径、热稳定性、乳化特性及流变学特性，以实验室选育的一株胞外多糖（EPS）高产菌株（Bacillus amyloliquefaciens LPL061）为材料，并考察EPS的体外免疫调节功能。结果表明：芽孢杆菌EPS粒径大小为445.6 nm；熔融温度为265.18 ℃；对食用油乳化效果较好，乳化指数达50%以上；EPS溶液为典型的假塑性流体，在20~80 ℃范围内黏度变化较小，具有较好耐温性；EPS能促进小鼠脾淋巴细胞增殖及细胞因子产生，具有一定的免疫活性。 |
| 关键词: 解淀粉芽孢杆菌 胞外多糖 理化特性 免疫调节 |
| DOI：10.11841/j.issn.1007-4333.2014.01.18 |
| 投稿时间：2013-04-30 |
| 基金项目:公益性行业（农业）科研专项经费项目（201303014-4）；国家自然科学基金资助项目（31271827）；国家大学生创新性实验计划项目（201210019088） |
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| Physicochemical properties and immunomodulatory effects of exopolysaccharide produced by Bacillus amyloliquefaciens |
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FAN Yi, LIU Li-sha, HAN Yu-zhu, LI Ping-lan
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| (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) |
| Abstract: |
| In this study,the particle size,thermal stability,rheological properties,emulsifying properties and immunomodulatory effects of exopolysaccharide of a high-yield strain Bacillus amyloliquefaciens LPL061 were studied and compared with those of xanthan gum.The results showed that the molecular size of EPS was 445.6 nm,the melting temperature is 265.18 ℃.LPL061 produced more stable emulsions with edible oils than xanthan gum.EPS also showed pseudoplastic non-Newtonian fluid behaviour in an aqueous solution,with thermal stability between 20 to 80 ℃.The EPS from LPL061 showed potential of immunomodulatory effects by promoted the lymphocyte proliferation of mouse. |
| Key words: Bacillus amyloliquefaciens Exopolysaccharides(EPS) physicochemical properties immunomodulatory |
