| 摘要: |
| 本研究通过分析在其他植物中已经确认的GA受体基因序列,对葡萄全基因组进行BLAST比对,获得了可能编码葡萄GA受体GID1的7个基因片段序列。经过聚类分析,根据其序列特征,分别设计特异性引物,以无核白鸡心葡萄(Vitis vinifera L.cv.Centennial seedless)不同组织器官为试材提取RNA,反转成cDNA-1链后,进行半定量RT-PCR,结果显示这些基因在成叶、幼叶、花序、新梢顶尖和休眠芽中存在差异表达。以30 mg/L的GA3在盛花后12 d处理无核白鸡心果穗,并于处理后1、3和7 d (果实第1次快速生长期),33 d (缓慢生长期),49 d (果实第2次快速生长期)进行采样,提取果实RNA,半定量RT-PCR结果显示除VvGID1-4和VvGID1-5外,果实中这些推测的GID1推测基因的表达水平在GA处理条件下均有上调或下调表达,推测上述研究结果为葡萄GA受体的全基因克隆与功能验证奠定了基础。 |
| 关键词: 葡萄 GA处理 GA 受体 半定量RT-PCR 差异表达 |
| DOI：10.11841/j.issn.1007-4333.2011.02.009 |
| 投稿时间：2010-06-12 |
| 基金项目:国家自然科学基金(30471212、30500347); 中国农业大学基本科研业务费资助项目(2009JS51) |
|
| Putative GA receptor gene family of Vitis vinifera L. andtheir differential expression under GA treatment |
|
GE Hui1, ZHAO Xiao1, Wang Zeng1, Chen Shang-wu2, MA Hui-qin1
|
| (1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China;2.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China) |
| Abstract: |
| Gibberellins (GA) are key plant hormone which promotes the enlargement of seedless grape berries.GA receptor and the pathway of GA signal transduction in grape berries are still unknown.Basing on sequence comparison of already isolated GA receptors from other plants,7 putative GA receptor GID1 genes were identified from the grape genome.They were analyzed with BLAST program,and specific primers were designed accordingly.Semi-quantitative RT-PCR showed that these 7 genes are of different transcription levels in mature leaves,young leaves,inflorescences,shoots and dormant buds.Bunches of Centennial seedless were treated with 30 mg/L GA3 on 12 days after flowering (DAF).Berried were collected at the first rapid growth stage (19 DAF),slow growth stage (45 DAF) and the second rapid growth stage (61 DAF).Semi-quantitative RT-PCR demonstrated that these putative GID1 genes,except VvGID1-4 and VvGID1-5,were down-regulated or up-regulated by GA treatment.Our results lay the foundation for future work on full sequence clone and functional study of grape GA receptors. |
| Key words: grape GA receptor semi-quantitative RT-PCR gene expression |
