| 摘要: |
| 为揭示鹅脂蛋白脂酶(LPL)脂质和肝素结合区域的特性,对鹅LPL基因外显子4~6进行了克隆和序列分析。以皖西白鹅血清总DNA为模板,设计引物对鹅LPL基因的第4~6外显子区域进行扩增和测序,分析鹅与其他物种之间该区域的DNA序列同源性、密码子非随机应用以及相应氨基酸残基区域的亲水性。结果表明,鹅LPL基因外显子4~6的片段大小分别为112、234和243 bp,其序列与鸡LPL基因相应序列的同源性达到91.9%,与人、羊、牛、猪等哺乳动物的同源性为74%~77%;鹅与鸡密码子非随机应用的相似系数为0.839,与猪和鼠的相似系数为0.580~0.650;LPL中由外显子4~6编码的氨基酸残基有4个区域具有较大的表面概率,分别位于残基145~148、残基187~190、残基255~257和残基293~299区域,表面概率分别1.62~4.29、2.06~3.01、2.75~4.46和3.03~6.01,这些区域伴随有较强的亲水性。 |
| 关键词: 皖西白鹅 脂蛋白脂酶 外显子 基因克隆 序列分析 |
| DOI：10.11841/j.issn.1007-4333.2007.05.096 |
| 修订日期：2006-05-29 |
| 基金项目:安徽省人才基金(人才-4);安徽省教育厅自然科学基金 |
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| Cloning and analyzing exon 4, 5 and 6 of lipoprotein lipase gene in goose |
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| Abstract: |
| Exon 4 to intron 6 sequence of lipoprotein lipase(LPL) gene was cloned to determine genome characteristics in goose.The total DNA from goose serum was extracted.Two pairs of primers,P and Q,were designed to amplify the LPL gene exon 4 to intron 6 which were then cloned and sequenced.The results showed that the LPL gene exon 4 to intron 6 were 3 404 bp.Blasting the sequence homology among chicken,human,pig,mouse,cattle and cat showed that the DNA homology between goose and chicken was 91.9% which was the highest between goose and other species.The homology between goose and mammal(human,sheep,cattle and pig) was 74 to 77%.Also the hydrophilicity,average charge,surface probability and condon usage of amino acides coded by LPL gene exon 4 to exon 6 in goose were analysed. |
| Key words: goose,lipoprotein lipase,exon,gene clone,sequence analysis |
