| 摘要: |
| 用实验室保存的24株乳酸菌菌种做了对人结肠腺癌细胞系HT-29细胞的体外粘附性试验。发现双歧杆菌的粘附能力较高,平均在 10.5个/细胞;乳杆菌的粘附能力较低,平均在 3.2个/细胞;球菌的粘附能力很低,平均在 2.3个/细胞。其中双歧杆菌中 Bifidobacterium bifidum 02菌株粘附能力最好,达到(18.4±2.7)个/细胞。乳杆菌中 Lactobacillus acidophilus 99101粘附能力最好,达到(10.2±1.8)个/细胞。同时,同种不同株的乳酸菌粘附能力有差异,而同属中,来自人粪便与来自猪粪便的乳酸菌的粘附能力没有明显差异。 |
| 关键词: 体外粘附试验 人结肠腺癌细胞 乳酸菌 HT－29细胞 活菌制剂 |
| DOI： |
| 修订日期：2001-07-05 |
| 基金项目: |
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| Study on Adhesion Ability of Lactic Acid Bacteria to the Colonic Adenocarcinoma Cell Line HT-29 |
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| Abstract: |
| 24 strains of lactic acid bacteria, including 11 strians of Bifidobacterium, 10 strains of Lactobacillus and 3 strains of Cocci, were selected and their adhering abilities to colonic adenocarcinoma cell line HT-29 were tested individually in vitro. The results showed that the mean adhesive levels (organisms/cell) of Bifidobacterium, Lactobacillus and Cocci to cell line HT-29 were 10. 5, 3. 2 and 2. 3 respectively. The most highly adhering strian in Bifidobacterium to cell line HT-29 was B. bifidum 02 and the adhering level was 18. 4?. 7; the most highly adhering strain in Lactobacillus was L. acidodophilus 99101 and adhering level was 10. 2?. 8. In addition, the different strains in the same species expressed various adhering capacities, and no obvious difference in adhering abilities were observed between the lactic acid bacteria which belongs to the same genus from faeces of pig and human. |
| Key words: lactic acid bacteria,adhesion,HT-29 cells |
