| 摘要: |
| 研究了蚕豆离休叶片失水10％后，叶片与其气孔保卫细胞脱落酸含量的变化及其与叶龄的关系。未受胁迫叶片脱落酸含量在成熟叶片中高于幼嫩叶片，保卫细胞中脱落酸含量均在很低的水平。在叶片自然失水10％的10min内，叶片的脱落酸含量几乎无变化，但保卫细胞的脱落酸含量增加5倍以上。4h后，叶片脱落酸含量也显著增加，从最幼嫩叶片到成熟叶片分别达到处理前的25，26，17，10和6倍。因是离休叶片，排除了脱落酸的其他来源，说明叶片在失去膨压时合成并积累脱落酸；所有保卫细胞脱落酸含量都增加30倍以上，以第2，3片完全展开叶片的保卫细胞最为敏感，其脱落酸含量增加40倍以上。失水处理后保持湿润恢复膨压的叶片和其保卫细胞的脱落酸含量没有增加。分析了在水分胁迫下保卫细胞中增加的脱落酸的可能来源。 |
| 关键词: 水分胁迫,脱落酸,保卫细胞,蚕豆 |
| DOI： |
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| 基金项目:国家攀登计划资助 |
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| Water Stress-Induced Abscisic Acid Accumulation in Detached Leaves and Guard Cells of Vicia faba L. |
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| Abstract: |
| Detached Vicia faba L. leaflets were water stressed. The ABA contents of leaves and guard cells were assayed in different time. The ABA contents of leaves were age-dependent in the unstressed leaves, the ABA level was higher in the mature leaves then younger leaves. There was a low level ABA in all guard cells. Within stress for 10 min, leaf ABA contents were not changed, but guard-cell ABA contents increased five folds. four hours after stress, ABA contents in leaves increased 25, 26, 17, 10, and 6 times respectively from the youngest leaves to mature leaves. It's suggested that leaves were synthesis ABA when isolated leaves lost their turgor. ABA contents in guard cells increased about 40 times. Four hours after stress relief, the ABA contents of leaves and guard cells were near the prestress value. The sources of increased ABA in the guard cells were disscussed. |
| Key words: water stress,abscisic acid,guard cell,Vicia faba L |
