| 摘要: |
| 以梯度SDS－PAGE方法分析了T型小麦雄性不育系及其保持系种子醇溶蛋白和谷蛋白组分，发现两系间有明显差异，而且四个不同品种小麦不育系均有各自与保持系区别的谱带出现，表明T型雄性不育小麦醇溶蛋白基因的表达有品种特异性。用单、双向电泳结合银染技术进一步分析了胚乳醇溶蛋白，发现不育系11A和14A，分别有24kD和36kD的特异蛋白。 |
| 关键词: 小麦,T型雄性不育,种子蛋白,双向电泳,梯度SDS－PAGE |
| DOI： |
|
| 基金项目:北京农业大学植物生理生化开放实验室资助 |
|
| Electrophoretic Analysis on Constitutions of Seed Protein from T-type Male Sterile Wheat |
|
|
|
| Abstract: |
| Differences have been found in wheat gliadins and glutenins of male-sterile lins and maintainer lines of T-type using silver-stained one and two-dimensional gel electrophoresis. A 36 kD polypeptide was found in male-sterile line of 14A. The male-sterile line of 11A showed a 24 kD polypeptide and the contents of 30 kD protein and 28 kD protein were much higher compared with its maintainer line.Gliadins of four different wheat cultivars were studied and results revealed that the differences between male-sterile lines and their maintainer lines were dependent on the cultivar. |
| Key words: wheat,T-type male sterility,seed protein,two-dimensional electrophoresis,gragient SDS-PAGE |
