| 摘要: |
| 通过对三个半矮生(brachytic—2,即 br2)玉米(Zea mays)及其高秆等基因同型系和相应的杂种 F1主要生育期过氧化物酶活性的测定,探讨了 br2基因的遗传矮化作用的化学机制。休眠种子过氧化物酶活性最低,种子萌发后,过氧化物酶活性迅速提高,但主要集中在种胚内;在不同器官中;根内酶活性最高,依次是叶、雌雄穗柄和叶鞘,节间的酶活性最低;根、叶、叶鞘表现为较老的组织酶活性高于新生组织,但节间的过氧化物酶活性,矮秆系以拔节期最高,高秆材料从拔节到蜡熟大致维持在稳定的水平;只有拔节期节间的酶活性高矮秆材料才表现显著差异,这种差异与成熟期株高呈显著负相关。我们设想:br2基因很可能仅仅在拔节期对伸长中的节间发挥作用,这种作用主要通过抑制吲哚乙酸(IAA)的代谢而阻碍细胞伸长,从而最终降低植株高度。 |
| 关键词: 玉米 不同器官 过氧化物酶活性 基因型 基因对 节间 矮化作用 遗传 拔节期 生育期 |
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| PEROXIDASE ACTIVITY AND THE GENETIC DWARFING OF GENE BR2 IN CORN (ZEA MAYS) |
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| Abstract: |
| Studies on corn were conducted to determine whether quantitative differences exist in the peroxidase activity of semidwarf and tall inbr eds and their corresponding hybrids,which are isogenic or near isogenic except one single gene(br2locus). peroxidase activity was determined for dormant seeds,germinating seeds,roots,Ieaves,Ieaf sheaths tassel peduncles,ear peduncles and internodes.Enzyme activity of dormant seeds was lower than that of germinating seeds.Embryo is the primary site of peroxidase.Peroxidase... |
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